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Organ culture and whole mount immunofluorescence staining of mouse Wolffian ducts

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journal contribution
posted on 2025-05-10, 13:45 authored by Manish Kumar, Pradeep TanwarPradeep Tanwar
We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

Funding

NHMRC

ARC

History

Journal title

Journal of Visualized Experiments (JoVE)

Issue

119

Publisher

Journal of Visualized Experiments

Language

  • en, English

College/Research Centre

Faculty of Health and Medicine

School

School of Biomedical Sciences and Pharmacy